Super-resolution techniques break the 200 nm limit on image resolution imposed by the diffraction of light. The Nanoimager achieves super-resolution by single-molecule localization (dSTORM and PALM). These techniques involve localizing only subsets of fluorophores in consecutive frames with high precision (typically better than 20 nm laterally and 50 nm axially), and reconstructing an image from the positions of the localizations.
The Nanoimager offers four laser lines with powers up to 1 W and the highest available power densities of any commercial instrument, calibrated at the sample plane and reported in real time. The two emission channels enable simultaneous dual-color imaging (four color imaging is possible through interlaced lasers). It supports colocalization studies and the capture of dynamic information for different molecular species.
3D information can be captured using the method of astigmatism for super-resolution detail, and by imaging sequential sections for greater depth.
The Nanoimager microscope unit has a footprint of just 21 cm by 21 cm.
The compact design is space-saving, but also reduces aberrations and loss of light in the optical path.
The Nanoimager geometry inherently compensates for drift. It houses specialist materials within the solid microscope body which significantly reduce thermal drift. Multiple software components further eliminate the effect of thermal drift. In addition to the anti-drift features, it has full vibration dampening which is supported by its compact design.
Together, this produces the most stable instrument on the market with no need for an optical table
The Nanoimager boasts the largest commercially available field of view, which is evenly illuminated throughout. The large field of view facilitates high-throughput imaging of single molecules and rapid accumulation of sample statistics.
As a Class 1 laser product, the Nanoimager can be safely operated in any room or laboratory.
The advanced sample stage has exceptional positional accuracy and reproducibility.
Autofocusing allows automated data acquisition over multiple fields of view and the rapid overview feature negates the need for oculars.
Whole-body heating to 37°C supports live-cell imaging and avoids the disruptive effect of temperature gradients.
Intelligent data analysis using our custom software suite is provided for all the Nanoimager modes of operation, including super-resolution imaging, smFRET and sptPALM.
Single-molecule imaging based 3D localization microscopy
Förster resonance energy transfer (FRET) spectroscopy
Lateral: exceeding 20 nm
Axial: exceeding 50 nm
Simultaneous imaging channels
2 (< 10 nm channel mapping accuracy)
Total number of imaging colors
50 μm x 80 μm per channel
Real-time 3D localization analysis and rendering (sCMOS optimized)
Real-time FRET trace analysis
Clustering and co-localization analysis
Residual drift correction
Scripting interface and OMERO compatibility
100 fps full frame
5 kHz with frame height cropped to 2%
Time for super-resolution full frame
Seconds to minutes (number of localizations and laser power dependent)
<1 μm/K drift
<1 nm vibration amplitude (1 Hz to 500 Hz)
Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection
Closed-loop adjustments of laser power density at sample plane
Resistive heating, whole instrument (for live cell imaging)
Sensor array (temperature, humidity, acceleration)
Microscope: 21 cm x 21 cm x 15 cm
Light engine: 21 cm x 42 cm x 45 cm
Latest generation sCMOS
82 % peak QE
1.6 electrons rms read noise at standard scan
Oil immersion, NA = 1.4 to NA = 1.49
Violet: 405 nm (150 mW)
Blue: 473 nm (300 or 1000 mW), 488 nm (200 mW)
Green: 532 nm (300 or 1000 mW), 561 nm (200 or 300 mW)
Red: 640 nm (300 or 1000 mW)
Near infrared: 730 nm (300 or 1000 mW)
LED for bright-field imaging
NIR auto-focus laser
20/20/10 mm XYZ travel range, closed-loop piezo stage with 1 nm encoder resolution
PC or laptop included (32GB RAM, nVidia GeForce GTX 1080/m)
Nanoimager software included, along with all future updates